Development of Gas-Exchange Measurement System for Micropropagated Plantlets Using 13CO2.

概要

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A new system was developed to measure photosynthetic and respiratory gas-exchange rates of micropropagated plantlets. In conventional photomixotrophic micropropagation, the proportion of carbon uptake from atmospheric CO2 and from sugar in the medium at the various growth stages is not clear. A method to directly measure CO2 uptake and release would help determine optimal culture conditions for micropropagated plantlets. The present system was designed to measure the amount of CO2 absorbed and released by the micropropagated plantlets by using stable isotope 13CO2 as a tracer gas. The system included an open gas flow type measurement vessel, equipment to control the composition of the gas supplied to the vessel, and a stable isotope mass spectrometer for the gas concentration measurements. The mass spectrometer could differentiate between 12CO2 and 13CO2, and measure the concentrations of both gases simultaneously in a continuous flow of gas. The use of these two forms of CO2 is effective for analyzing the flows of CO2 from photosynthesis and respiration. Sweetpotato plantlets (Ipomoea batatas (L.) Lam.) 'Beniazuma', 28 days after subculture, were used for the measurements. As the first part of the study, the changes in 13CO2 concentration during photosynthesis and dark respiration were examined.
日本農業気象学会の論文