Precise Evaluation of Enzyme Activity using Isothermal Titration Calorimetry

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概要

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• Isothermal titration Calorimetry (ITC) has become a powerful method for evaluating enzyme kinetics, as it provides a way to detect catalytic reaction heat as a function of time with high sensitivity and reproducibility. This review focuses on both established and newly emerging methodologies for evaluating the kinetics of enzyme-catalyzed reactions. Because it allows direct determination of the reaction rate, which itself indicates enzyme activity, ITC is expected to provide a general and effective way to evaluate enzyme activity. The theoretical basis of ITC for evaluating enzyme kinetics is reviewed, as demonstrates the advantages of this method.

• 日本熱測定学会の論文

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