The helix-coil transition of plasmid DNA and genetic function.
スポンサーリンク
概要
- 論文の詳細を見る
Differential scanning calorimetry (DSC) of several plasmid DNAs was carried out. The DSC curves for the DNAs linearized with a restriction enzyme provided several peaks due to the stepwise transition of double-stranded DNA to single-stranded DNA along the molecular chain. These DSC curves are very similar to the melting profiles constructed by calculation from the entire base sequences of these plasmids according to the helix-coil transition theory. The theoretical analysis shows that the boundaries of cooperative melting regions of the DNA coincide with the end regions of each of the coding regions. The interaction between various drugs and plasmid DNA was also studied by the DSC method. The DSC curves of DNA in the presence of drugs are affected in the characteristic manner depending upon the interaction mode of the drug, demonstrating that DSC method is very useful for the study of the drug-DNA interaction.
- 日本熱測定学会の論文
日本熱測定学会 | 論文
- CALPHAD法援用による合金のアモルファス形成能評価
- 薬物と血清アルブミンの相互作用
- DCNQI-Cu系リエントラント金属-絶縁体転移の熱的研究
- サーモトロピック液晶とリオトロピック液晶--統一的視点の可能性
- 大学における熱測定教育の意義と実験教材 (特集 熱力学・熱測定と教育)