A study on the effectiveness of pulmonary flushing for lung preservation
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概要
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The effect of pulmonary flushing prior to lung preservation was studied using an <I>ex vivo</I> perfusing rabbit model. The grafts were divided into the following five groups : Group I ; surface cooling without flushing (n=6), Group II ; flushed <I>in vivo</I> with intracellular solution (A solution) (n = 6), Group III ; flushed <I>ex vivo</I> with A solution (n = 6), Group IV ;flushed <I>in vivo</I> with extracellular (B solution) (n=6), Group V : flushed <I>ex vivo</I> with B solution (n=6). After 18 hours' preservation at 8°C, the function of preserved grafts was examined for 60 minutes with a closed circuit system.<BR>In flushing with A solution, the pressure increased after 2 minutes (p <0.01) and the perfusion volume decreased after 4 minutes (p<0.01), while the flow of the B solution groups was constant throughout. The cooling effect, shown by the effluent temperature, and the flushing effect, measured in the <I>in vivo</I> group compared with the <I>ex vivo</I> groups (p<0.050.01). The flushing of Group V was most effective from the three points of view of perfusion, cooling and washing out.<BR>In the functional assessment of preserved grafts, Groups, II, III and IV dropped out due to permeability injury within 20 minute. Group V had better graft function in blood gas data, perfusion pressure, tidal volume, the perfusate decrement, and wet-dry ration (p < 0.050.01), compared with Group I.<BR>We conclude that the <I>ex vivo</I> flushing with extracellular solution in the best lung preservation method, and is more useful than surface cooling only.
- 特定非営利活動法人 日本呼吸器外科学会の論文