Preparation of Liposomal Ferromagnetic Particles and Application of High Frequency Magnetic Fields

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Lipid- filmed magnetite particles associated with tumor-localizing hematoporphyrin (hematoporphy rin liposomal magnetite : HP-LM) were prepared by sonication of hematoporphyrindimethylester-as-sociated liposome solution with ferromagnetic magnetite (Fe<SUB>3</SUB>O<SUB>4</SUB>) particles (ca. 150 Å). The HP-LM solution was fully suspended. HP-LM is expected to be small enough to get through the blood capillary and to have no immunogenicity. The HP-LM solution can be inductively heated by the magnetic hysteresis loss with the application of a high frequency magnetic field (HFMF). Temperature rises, with applications of HFMF (2MHz), of the HP-LM solution and the homogenized mouse liver tissue with addition of HP-LM showed linear increase with HP- LM concentrations. BALB/c mice implanted with Meth- A tumor were intravenously administrated with HP- LM and HP- free LM. Removed, at 24, 48 and 72 hours after administration, tumors and tissues (liver, kidneys, spleen and lungs) were applied with HFMF (2MHz), and temperature rises were measured. Tumor tissues of the mice administrated with LM's, especially with HP- LM, had some temperature rises, which suggested that LM's were distributed to tumor tissues. Liver and spleen, as well, of the mice administrated with LM's had high temperature rises, showing that LM's were highly uptaken by the reticuloendothelial system.
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