Effect of inhaled nitric oxide on oxygen-induced DNA damage.
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概要
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To determine whether inhaled nitric oxide (NO) at a concentration of 20 parts per million (ppm) attenuates or enhances oxygen-induced lung injury, the effect of inhaled NO on the formation of 8-hydroxydeoxyguanosine (8-OHdG), a marker for oxidative DNA damage, was studied in rat lungs. Forty male Wister rats were divided into three groups; 20 rats in the control group, 10 rats in the oxygen group, and 10 rats in the oxygen-NO group were exposed either to room air, 100% oxygen, or oxygen with 20ppm NO for 48 hours. Their lungs were removed and homogenized. DNA was extracted to measure 8-OHdG/dG×105. In the control group, 8-OHdG/dG×105 was 1.02±0.35. Exposure to either 100% oxygen or oxygen with 20ppm NO caused significant increase in 8-OHdG/dG×105 from 1.02±0.35 to 1.77±0.74 (P<0.01) or to 1.37±0.55 (P<0.05), respectively. Although statistically not significant (P=0.09), the magnitude of increase in 8-OHdG/dG×105 was lower in the oxygen-NO group than that in the oxygen group. These results suggest that inhalation of NO does not enhance the oxidative injury of DNA in rat lungs.
- 一般社団法人 日本集中治療医学会の論文
一般社団法人 日本集中治療医学会 | 論文
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