Eradication of the melon fly from Kikai island by the application of the sterile insect release method. 4. Methods for marking and transportation of sterilized pupae.:4. Methods for marking and transportation of sterilized pupae

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To eradicate the melon fly, Dacus cucurbitae COQUILLETT, from Kikai Island by the sterile insect release method, procedures for marking and transportation of sterilized pupae were tested. The normal eclosion of adults was adversely affected by the shaking of pupae for 15 to 45 sec to mark them with a fluorescent dye. A volume of ten thousand pupae (Y) could be estimated by the number of pupae weighing 5g (X) based on the following equation, Y=-0.946X+605.652 (r=-0.8204). This equation enabled to determine the number of pupae per shipping envelope. The pupae marked with the fluorescent dye and packed in the shipping envelopes were loaded on a truck with a refrigerated container, and the truck was transported by a ferry boat from Naze to Kikai Island. The pupae were kept for about two days in the refrigerated container. By controlling the temperature of the container, the temperature inside the shipping envelope could be adjusted in the range of 20 and 30°C ; these conditions were considered to be favorable for the normal eclosion of the pupae.
九州病害虫研究会の論文