Some properties of the virus isolated from broad bean showing necrotic mosaic in Ehime Prefecture

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Some properties of the virus isolated from the broad bean leaves showing necrotic mosaic in Ehime Prefecture were elucidated. The electron microscopic observations carried out by means of dip mathod revealed that the virus particles were rod-shaped with round tip at one end showing two predominant lengths, 130-150 nm and 230-250 nm. Both particles were 25 nm in width. The virus caused necrotic mosaic symptom on broad bean and pea, and local lesions on Chenopodium amaranticolor and C. quinoa. No symptom was observed on sweet pea, bean, Nicotiana clevelandii and N. rustica and virus was not recovered from these plants. Inclusion bodies showing net-like membranous structure were found frequently in the cytoplasm of the infected broad bean leaf cells.<BR>The soil transmission tests conducted in glass house at 15 C, 17 C and 25 C by using virus-contaminated soil collected from natural field were unsuccessful, however, it was successful when broad beans were sawn outdoors from October to November. These results suggest that this virus seems to be a strain of broad bean necrosis virus.
九州病害虫研究会の論文