A Study on a Fractional Elution Method in Paper Electrophoresis
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概要
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In view of the complexity and inaccuracy of densitometery now in use, we adopted a fractional elution method as a substitute for it and made basic studies on the same with the following results:1. Pigments are completely extracted from pieces of filter paper with 5-10cc of N/100 NaOH solution in 30 minutes at room temperature. The extinction of the extract and the amount of serum added show a linear relationship.2. The color tone of the extract, which in prepared by removing bromphenol blue (BPB) or Amidoschwarz 10B (AS) from filter paper with N/100 NaOH solution, fades away with the passage of time; this phenomenon is especially remarkable in the extract which is prepared by removing BPB from filter paper with the said solution. Since the tendency of the color tone to fade away is true of each fraction of serum protein, no significant differences take place at least for 5 hours when the percentage of protein in serum is calculated.3. The maximum resorption wavelengths of the extracts which are prepared by removing BPB and As from filter paper with N/100 NaOH solution are 570 and 620mμ.4. When measurement is made by only an individual according to this method, its reproductivity is considerably high in each serum of healthy subjects and cases of nephrosis and liver cirrhosis.5. Even when this method is performed on different individuals, it shows a reproductivity equal to that of the Tiselius method.6. By this method we followed great changes in serum protein in cases of nephrotic syndrome that were produced during a short time by a large dosage of predonine.
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