Investigation for new biomarker of primary esophageal squamous cell carcinoma using two-dimensional differential gel electrophoresis

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Recent advances in 2-D differential gel electrophoresis (2-D DIGE) have made it possible to detect and quantitate the critical changes involved in disease pathogenesis. We have identified novel proteins with altered expression in primary esophageal cancer using the powerful method of agarose 2-D DIGE. Excised tissues from 12 patients of primary esophageal cancer were obtained. Proteins with altered expression between cancer and adjacent non-cancer tissues were analyzed by agarose 2-D DIGE and identified by mass spectrometry. 2-D DIGE has many advantages on proteomic analysis for clinical specimens. 33 proteins with altered expression in tumors were identified. Among them, a 195kDa protein, periplakin, was significantly downregulated in esophageal cancer, which was confirmed by immunoblotting. Immunohistochemistry showed that periplakin was mainly localized at cell-cell boundaries in normal epithelium and dysplastic lesions, while it disappeared from cell boundaries, shifted to cytoplasm, in early cancers and less expressed in advanced cancers. These results suggest that periplakin could be a useful marker for detection of early esophageal cancer and evaluation of tumor invasion, metastasis and progression.
日本電気泳動学会の論文