Glycosylation analysis of glycoproteins by LC/MS/MS: analysis of glycosylation sites and of site-specific heterogeneity.:analysis of glycosylation sites and of site-specific heterogeneity
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概要
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Liquid chromatography/tandem mass spectrometry (LC/MS/MS) is a powerful tool for the analysis of glycosylation sites and of site-specific glycosylation in a glycoprotein. The glycopeptides in a complex mixture of tryptic digest can be separated and monitored by using oxonium ions produced from a carbohydrate moiety through CID-MS/MS. Based on b and y ions in the product ion mass spectra, peptides can be identified, and the structure of carbohydrates can be deduced from B ions and the molecular weight of precursor glycopeptide. Here we show the site-specific glycosylation analysis of α-fetoprotein and an SDSPAGE gel-separated GPI-anchored protein.
- 日本電気泳動学会の論文
日本電気泳動学会 | 論文
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