N-terminal amino acid sequences of cashmere fiber protein in the Korean native goat.

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The expression level of guard hair fiber (following hair fiber) protein in the Korean native goat showed remarkably seasonally change. The protein components (M.W.: 9kDa) specific for the cashmere fiber were isolated. To determine the N-terminal amino acid sequences on five kinds of major proteins for hair fiber and cashmere fiber, protein components (50 and 42kDa) of the hair fiber which showed seasonal change in the level of protein expression were determined as T-L-G-F-Y-T-A-G-P-A-F-L-L-V---F-Q-K and I-L-N-G-T-R-T-R-P-F-R-F-I-I-P, respectively. And this 42kDa protein component had the homology (61.5%) with integrase. A common protein (17kDa) having sequence of T-G-S-C-C-G-P-T-F between the hair fiber and the cashmere fiber was identified as a keratin protein. N-terminal amino acid sequences of cashmere fiber proteins with 50kDa and 42kDa, were determined as T-L-G-F-Y-T-A-G-P-A-F-L-L-V and I-L-N-G-T-------P-F, respectively, and the sequence of 17kDa keratin protein was determined as T-G-S-C-C-G-P-T-F-S. Also that of 9kDa, which is specific for the cashmere fiber protein was determined as T-G-F-T-(G)-G-G-I-Y-F-P-G---T, which might be the novel protein component.
日本電気泳動学会の論文