New developed fluorescent differential display method.
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概要
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In many applications, an understanding of differentially expressed genes in different tissues or owing to an applied stimulus is important. However, the wide use of polymerase chain reaction (PCR)-based techniques for the identification of differentially expressed mRNAs (RNA fingerprinting by arbitrarily primed PCR) and differential display (DD) has shown that using radioisotopes is still a problem. Although a technique has been developed that avoids some of the disadvantages, the use of radioisotopes for band detection still limits various applications. It has been improved this technique for analyzing differentially expressed mRNA by resolving the amplified products using fluorescent-labeling as a non-radioisotope. Our modified method allows the improvement of identification on differentially expressed bands with a high accuracy. This technique with a non-radioisotope representation may be possible to perform PCR-DD analysis with many applications.
- 日本電気泳動学会の論文
日本電気泳動学会 | 論文
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