Improvement of the standard electrophoresis procedure for serum protein fractions. effect of constant current and constant voltage on cellulose acetate membrane electrophoresis using Separax SP.:effect of constant current and constant voltage on cellulose

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Cellulose acetate membrane electrophoresis was performed at a constant current and constant voltage using a new cellulose acetate membrane, Separax SP, which is slightly electro-osmotic. The rate of evaporation flow was obtained by observing the movement of Vitamin B12 which had been applied to the cellulose acetate membrane. Evaporation flow was smallest in the inner part of the membrane of electrophoretic axis and largest towards the outer part. The evaporation rate was not affected by changes in the current or voltage. However, it was dependent on the total power. Separax SP is usually considered not to be electro-osmotic. However, application of an alternating current revealed electro-osmotic properties. Even when total electrical power was kept constant, the electrophoretic patterns of the serum proteins differed, depending on whether we used constant current or constant voltage for run. These differences are attributable to electrical changes, during electrophoresis. With constant voltage, a small amount of electricity is applied during the early stages of electrophoresis and a large amount of electricity is the finishing stages. This provides a better separation of the samples.
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