Capillary electrophoretic separation of human serum proteins by the addition of trimethylammoniumpropanesulfonate(Z1-methyl) to the buffer.
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概要
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Recent data have demonstrated that a capillary electrophoresis (CE) using untreated fused silica capillary has some advantages for separation of proteins or other substances. It is, however, necessary to minimize nonspecific adsorption of proteins to the wall, which cause decreased reproducibility. In order to overcome this problem, we extended a CE method by adding trimethylammoniumpropanesulfonate (Z1-methyl) as a zwitterion to running buffer, where the pH was raised above the isoelectric points of sample proteins. We also checked the optical condition for the separation of human serum proteins. The results showed that the use of 50mM sodium borate (pH 10.0) including 1.0M Z1-methyl gave a reproducible and more evident separation pattern at the analysis of human serum proteins. These results indicate that CE with Z1-methyl was useful method on a clinical laboratory.
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