Electrophoretic analysis of suppression of gene expression with an antisense-ribozyme.
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概要
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We designed a ribozyme (catalytic RNA) to site specifically cleave the mRNA of the activated H-ras gene expressed in human bladder carcinoma EJ cells, human melanoma FEX-1 cells and activated H-ras transformed NIH3T3 cells. The optimal conditions for catalytic cleavage by the ribozyme were determined in vitro. A synthetic DNA encoding the ribozyme was cloned into a mammalian expression vector (pHβ Apr-1) and transfected into activated H-ras cells. Cells expressing the H-ras ribozyme were characterized by a marked reduction of H-ras gene expression. These results suggest the possibility of anti-oncogens ribozyme as suppressors of tumors.
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