Electrophoretic analysis of urinary proteins in domestic animals with acid violet 17 staining.
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Basic conditions of acid violet 17 staining was examined on the cellulose acetate membrane electrophoresis for domestic animals. Staining sensitivity of acid violet 17 was approximately 20 times more than ponceau 3R staining. This higher sensitivity allowed us to analyze specimens of urine at the concentration of protein as low as 40mg/dl. Acid violet 17 was a useful staining for the serum protein of cattle, pigs, dogs and chickens, but not for rabbits. The urinary proteins in healthy dogs and dogs with proteinuria were also demonstrated using the staining method with acid violet 17. According to the result, urine of healthy dogs contained 4∼14mg/dl protein, and urinary protein can be separated, albumin (14.4%), α∼β1-globulin (68.0%) and β2∼γ1-globulin (17.2%) by cellulose acetate membrane electrophoresis. The electrophoretic pattern of the dogs with proteinuria resembled with those of serum protein, indicating high contents of albumin. In addition, the urinary proteins of dogs with microfilaremia were characterized by a high content of β1∼γ-globulin and low molecular weight protein.
- 日本電気泳動学会の論文
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