Detection of the individual difference in the human seminal plasma analyzed by M2D-PAGE and CITP.
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概要
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For the purpose of the paper was to search for markers for individual identification of human seminal plasma samples by micro two-dimensional polyacrylamide gel electrophoresis (M2D-PAGE) and capillary isotachophoresis (CITP). The coomassie-stained M2D-PAGE patterns of seminal plasma protein showed the individual difference in the spots of Acp, Alb, Tf and γ-Sm. The individual differences in UV absorbance peaks in CITP patterns were correlated with those spots in M2D-PAGE patterns. Thirteen spots were detected in the zone of lower molecular weight around 5, 000 by silver staining after M2D-PAGE in the gradient of 6 to 25% for the second dimensional development. Three of the spots named low molecular weight seminal plasma protein showed the individual difference, Six phenotypes were detected in the human seminal plasma.
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