Modification of the estimating method of cardiac structural protein by two-dimensional electrophoresis and application to the analysis of structural proteins in left ventricular hypertrophy.
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概要
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The quantitative changes in the structural proteins of myocardial hypertrophy have not been known well.We modified the quantitative method of the structural protein using the two-dimensional electrophoresis and the pyridine extraction, and analyzed the changes of myocardium.As experimental animal, we used three models of left ventricular hypertension consisted of spontaneously hypertensive rats, aortic-constricted rats and two-kidney one clip hypertensive rats.It was usually difficult to analyze high molecular proteins according to the first dimension electrophoresis, so that we used agarose instead of polyacrylamide in the first dimension and homogenized cardiac muscle with 8M guanidine hydrochloride without the degradation of proteins.Because of the simultaneous determination of the major and minor components on a slab gel, we analyzed the coloring extracts from spots stained by 25% pyridine.
- 日本電気泳動学会の論文
日本電気泳動学会 | 論文
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