Development of a high sensitive method for specific detection of micro-heterogeneous components of tissue kallikrein. A method of direct detection on cellulose acetate membrane after isoelectric focusing.:A method of direct detection on cellulose acetate
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概要
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In this report, a new convenient method for detecting (micro-) heterogeneous components of tissue kallikrein with high sensitivity has been demonstrated. This method consists of an isoelectric focusing on cellulose acetate membrane and a direct immunodetection of kallikrein bands on cellulose acetate membrane using anti-tissue kallikrein anti-rabbit serum without any blotting technics. This method is specific for tissue kallikrein and has a high sensitivity for detecting (micro-) heterogeneous components at below 10ng of each one. This method also can detect (micro-) heterogeneous components of tissue prokallikrein, because anti-tissue kallikrein rabbit serum can react with tissue prokallikrein.
- 日本電気泳動学会の論文
日本電気泳動学会 | 論文
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