A procedure for the purification of ferritin using Matrex Red A.
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概要
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Dyematrex gel was used for the purification of ferritin. Rat liver ferritin was purified by a combination of heating of tissue homogenate, ammonium sulfate precipitation and Sephacryl S-300 gel filtration as usual and finally applied on Matrex Red A column equilibrated with 20mM Tris HCl buffer, pH 7.5. All several contaminated proteins were adsorbed on Matrex Red A and eluted by 1.5M KCl containing the buffer. Ferritin passed through the column and the recovery was over 90% of an applied amount. Finally purified preparation was observed as single band on 7.5% polyacrylamide gel with silver stain and showed the same isoferritin pattern before the use of Matrex Red A. Acidic ferritin was also purified simply at high yield from human hepatocellular carcinoma tissue by the same procedure.
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