Analysis of human seminal proteins by micro two dimensional electrophoresis. (Part II).
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概要
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Human seminal proteins were subjected to two-dimensional electrophoresis (isoelectric focusing/PAGE) under native conditions followed by CBB staining. 54 specific antisera for human plasma proteins and seminal proteins were used as primary antibody in ABC immunoperoxidase staining procedure after electro blot to membranes. The locations of human seminal proteins were determined on these blots using the specific antisera, and an "Identification map" was prepared. By this methods, prothrombin, fibrinogen, factor-12 and plasminogen of coagulation factor were detected in human seminal proteins.
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