Analysis of human seminal proteins by micro two dimensional electrophoresis. (Part I).
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概要
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Proteins in 70 human seminal fluids were analyzed by micro two dimensional polyacrylamide gel electrophoresis without denaturant and compared with respective serum proteins. Seminal proteins were separated into more than 80 protein spots. Thirty nine proteins under the molecular weight of albumin (6.8K) were found to be absent in serum proteins. One of these proteins was identified as γ-seminoprotein by using ABC method of a immunochemical staining after electro-blot to a membrane. As compared with the serum proteins, human seminal proteins were characterized by a number of spots which were unidentified proteins of low molecular weight.
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