Pepsinogen gene regulation during stomach development and in carcinogen induced stomach cancer.

概要

論文の詳細を見る
In order to understand the mechanisms involved in the regulation of pepsinogen synthesis, we have investigated the changes of the expression and methylation of pepsinogen 1 (Pg1) genes during stomach development and also in carcinogen induced rat stomach cancers. Restriction analysis using methylation-sensitive restriction enzymes revealed progressive demethylation changes in Pg 1 genes region that almost coincided with the progressive increases in Pg 1 mRNA expression and Pg 1 protein synthesis during stomach development. Thus, there was an inverse correlation between the expression and methylation of Pg 1 genes and some control mechanism utilizing DNA methylation was suggested in the regulation of Pg 1 genes expression. In MNNG-induced stomach cancer, most of the composing cells were non-Pg 1 producing and there was no Pg 1 mRNA. The methylation patterns of Pg 1 genes in these cancers were different from those of normal tissues which do and do not synthesize Pg 1. This finding is in good agreement with the previous finding that DNA methylation is altered in cancer cells.
日本電気泳動学会の論文