Isotachophoresis of monoclonal gammopathy(IgG type) serum.
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概要
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In order to evaluate the improved technique of capillary isotachophoresis, human sera from 10 normal subjects and 22 patients of monoclonal gammopathy (IgG type) were analyzed. The value of potential gradient was used to represent the mobility of each UV absorbing peak. All the sera from the patients were characterized by one or a series of sharp UV absorbing peak, which was at least 3-fold higher than the corresponding IgG peak of normal subject. Comparing the results with those obtained by polyacrylamide micro two-dimensional electrophoresis, it was as certained that the value of potential gradient of a monoclonal IgG molecule is related to its isoelectric point. Because of the highly simplified operation and the high resolution of proteins, the technique is promising for the routine analysis of serum proteins in clinical laboratories.
- 日本電気泳動学会の論文
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