Comparative analysis of proteins from albumen glands and eggs of land snail, Euhadra, using two dimensional gel electrophoresis.
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概要
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The soluble proteins extracted from albumen glands and eggs of land snails, Euhadra peliomphala, E. subnimbosa and E. quaesita in feeding, were analyzed using SDS polyacrylamide gel electrophoresis (SDS-PAGE) and O'Farrell's two dimensional gel electrophoresis (2D-PAGE).Although SDS-PAGE did not show any clear differences of the protein composition in three species, species specifc polypeptides were detectable by the use of 2D-PAGE. Moreover, 2D-PAGE patterns showed that albumen glands and eggs shared most of the major polypeptide spots.On the basis of the polypeptide mapping methods, all the polypeptides in albumen glands and in eggs were classified into 14 groups. The common polypeptide groups (13 groups of them) in both albumen glands and eggs, that might migrate from albumen glands to eggs during oogenesis, were demonstrated on the polypeptide maps.
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