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We established a simple method for measuring HDL subfraction cholesterols by gradient polyacrylamide gel electrophoresis (gradient PAGE). Prestained human serum was separated into two major bands identical with HDL2 and HDL3 by the gradient PAGE. HDL-cholesterol (HDL-C) obtained by precipitation method was divided according to HDL2/HDL3 ratio determined by densitometry of the electrophoretic pattern. HDL subfraction cholesterols determined by this method was well correlated with those measured by zonal ultracentrifugation (HDL2-C: r=0.98, HDL3-C: r=0.94, n=11). Mean serum HDL2-C level was significantly higher in normal females than in normal males (p<0.01). HDL3-C levels were significantly decreased in liver diseases, especially in cirrhotics. This simple electrophoretic method for quantification of HDL2 and HDL3 is suitable for clinical application in various pathological states.
- 日本電気泳動学会の論文
日本電気泳動学会 | 論文
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