Electrophoresis of the glycosaminoglycans from the abdominal walls of the normal rats
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概要
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A study has been made of the glycosaminoglycans and glycopeptide in the abdominal walls of the normal rats. The abdominal walls were separated into three layers; skin, adipose tissue and muscle containing fascia, and then uronic acid, sulfate, hexose and sialic acid contents were measured in each layer. Values per g of wet tissue were highest in the dermis, while those of dry tissue were highest in the adipose tissue. Cellulose acetate strip-electrophoresis of concentrates in veronal butter revealed the presence of three alcian blue positive fractions in every layer, numbered in order of increasing electrophoretic mobility, fraction I, fraction II and fraction III; that is, fraction I corresponded in mobility to hyaluronic acid, fraction II to heparan sulfate, and fraction III to dermatan sulfate. The relative proportions of the fraction I, II and III were approximately 40%, 10% and 50%, respectively.
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