Studies on lactate dehydrogenase in adult female rabbit genital organs
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概要
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The total activity of lactate dehydrogenese (LDH) and LDH isozyme patterns were investigated in adult female rabbit genital organs before and after implantation. The animals were divided into four groups, namely follicular, ovulation, pre-implantation and implantation stage group. The results obtained are as follows.1. The LDH activity in Fallopian tube, uterus and ovary was lowest in follicular and implantation stage group, and higher in ovulation stage group and highest in pre-implantation stage group.2. The LDH activity in plasma, on the contrary, showed minimum value in pre-implantation stage group.3. The LDH activity of pre-implantation stage was three times as high as that of ovulation stage group in Fallopian tube and uterus.4. The LDH activity of Fallopian tube was twice as high as that of uterus in all four groups.5. LDH isozyme analysis by agar electrophoresis revealed that the LDH isozyme pattern in Fallopian tube was LDH5 type and LDH isozyme pattern in uterus was LDH1 type.6. There were no definite differences in LDH isozyme patterns among four groups.7. The LDH isozyme pattern in plasma showed LDH1 type in follicular and pre-implantation stage group, and LDH5 type in ovulation and implantation stage group.
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