Study of measuring microquantities of protein by immunoelectrodiffusion
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概要
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Immunoelectrodiffusion is a sensitive technique in measuring micro-quantities of proteins. In this method antigen is forced to flow into the gel containing antibody by electrophoresis.Agar gel antibody plates (7.5×7.5×0.1cm) containing 0.75ml of anti-γ chain rabbit serum were used in the present study to quantitate IgG globulin. Electrophoresis was carried out for 2 to 3 hours at 150V. The length of precipitate line thus formed was in proportion to the concentration of applied IgG in the range between 1.18 and 9.5mg/dl.The reported immunological technique is suitable for measuring micro-quantities of IgG in dilute solutions such as cerebrospinal fluid, urine and saliva without concentrating procedure.
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