Ultraviolet analysis of human serum proteins in zone electrophoresis
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概要
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Specific absorption coefficients of electrophoretically separated human serum protein fractions were determined. Fresh normal sera from 5 healthy blood donors were pooled and submitted to starch grain zone electrophoresis using either phosphate buffer at pH8.0 or veronal buffer at pH8.6. Protein fractions were eluted with neutral saline and optical density at 280mμ and nitrogen contents were determined. Specific absorption coefficient, ε, at 280mμ was expressed as O. D. of the solution of each protein fraction at a concentration of 1mg protein nitrogen per ml measured in a 1cm cell.Average values of ε for serum protein fractions obtained from six runs of electrophoresis with the phosphate buffer and six runs with the veronal buffer were as follows: albumin; 4.2, α-globulin; 6.7, β-globulin; 8.3, and γ-globulin; 9.5.Results obtained by densitometric analysis at 280mμ of agar electrophoretic patterns of human sera were corrected for the above mentioned specific absorption coefficients by multiplying the optical densities of albumin, α-, β-, and γ-globulins by 16, 10, 8, and 7, respectively. Percentage value of each serum protein fraction thus obtained were found to be in good agreement with that obtained by free electrophoresis in 10 cases of normal and 6 cases of pathological sera.
- 日本電気泳動学会の論文
日本電気泳動学会 | 論文
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