Strongly Anionic Sites in Peripheral Axons of the Rat Sciatic Nerve: Light and Electron Microscopic Detection Using Cationic Colloidal Iron.
スポンサーリンク
概要
- 論文の詳細を見る
Anionic sites in the rat sciatic nerve were studied by light and electron microscopy using a fine-granular cationic colloidal iron staining method (MURAKAMI et al., 1986). The axon, as well as the endoneurium, the epineurium and the basement membrane of Schwann cells, were all confirmed to react strongly to the cationic colloidal iron even at a pH value of 1.0-2.0. Prior hyaluronidase digestion decreased the colloidal stain of the epineurium; chondroitinase ABC weakened that of the endoneurium and the basement membrane of Schwann cells. However, as axons retained stainability with cationic colloidal iron even after combined digestion with hyaluronidase, chondroitinase ABC, heparitinase and keratanase, the authors consider sulfated glycoconjugates and not those substances which are digestible with such common enzymes. The acid groups ionized at pH 1.0 are most likely sulfate groups. Methylation deprived the axon of the reactivity to cationic colloidal iron staining, and even subsequent saponification could not recover this reactivity to its full extent. All these suggested the presence of sulfate groups. In the axon, electron microscopy revealed a deposition of colloidal iron on the external surfaces of microtubules and neurofilaments in the axoplasm and of very fine filaments connecting them. This highly negatively charged intra-axonal network could also serve toward a supportive function in maintaining the spatial distribution of microtubules either mechanically or through electrostatic repulsion or, possibly, serve as an intra-axonal cation exchange reservoir.
- 国際組織細胞学会の論文
国際組織細胞学会 | 論文
- Immunohistochemical localization of vascular endothelial growth factor in the endocrine glands of the rat
- Reduced expression of endogenous secretory receptor for advanced glycation endproducts in hippocampal neurons of Alzheimer’s disease brains
- A longitudinal study on the expression of the opsin gene in the degenerating retina of C3H/He mice.
- Expression of the arylhydrocarbon receptor in the periimplantation period of the mouse uterus and the impact of dioxin on mouse implantation
- Immunohistochemical studies on the development of tyrosine hydroxylase- and serotonin-immunoreactive neurons in fetal dorsal raphe tissue transplanted into the anterior eye chamber of adult rats.