Histological Localization of the Sweet Taste Receptor in Rat Taste Buds by the Use of Gurmarin, a Sweet Taste-Suppressing Peptide.
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The binding site of gurmarin, a peptide inhibiting the sweet-taste sensation, was studied in taste buds in rat circumvallate papillae by means of a histochemical technique. Frozen sections of tongues were incubated with gurmarin conjugated with biotin and thereafter examined with a light microscope. Positive reactivity for the peptide was localized to the taste hairs, the apical projections of taste bud cells. The reaction appeared in about 10% of the circumvallate taste buds examined. As electrophysiological studies indicate that gurmarin suppresses the sweet-taste sensation at the level of reception, the present study suggests that the receptor for sweet taste is located on the taste hairs and, furthermore, is present only in a certain, limited number of the taste buds.
- 国際組織細胞学会の論文
国際組織細胞学会 | 論文
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