Studies on intestinal absorption of .BETA.-galactosidase in neonatal and young adult rats.
スポンサーリンク
概要
- 論文の詳細を見る
The intestinal absorption of tilactase (containing 6.7% β-galactosidase, 10, 000 ONPG unit/g) was studied following oral administration to rats of different age(1-, 7-, 14-, 17-, 21- and 49-days old). Concentrations of β-galactosidase in plasma, tissues and other body fluids were detected by radioimmunoassay(RIA) and/or o-Nitrophenyl-β-D-gactopyranoside (ONPG) methods.<BR>1. After oral administration of tilactase at a dose of 4g/kg (β-galactosidase : 268mg/kg) to 49-days old rats, β-galactosidase was not detected in the plasma, liver and urine.<BR>2. After oral administration of tilactase at a dose of 0.04 and 0.4g/kg to 7-days old rats, traces of β-galactosidase were detected in the liver.<BR>3. After oral administration of tilactase at a dose of 4g/kg to 7-days old rats, the absorption of β-galactosidase from small intestine was observed with an estimated absorption ratio of 0.2%.<BR>4. After intravenous injection of β-galactosidase (1.15mg/kg) to 7-days old rats, high level was especially observed in the liver and spleen. In the case of high dose (β-galactosidase 11.5mg/kg), hepatic uptake was saturated.<BR> 5. β-galactosidase-like immunoreactive substance, which was absorbed from small intestine of 7-days old rats, was found to have similar enzymatic activity and molecular size as that of administered β-galactosidase.<BR>6. After intravenous injection to 7-days old rats, β-galactosidase was not excreted into bile and urine.<BR>7. After intravenous injection of <SUP>125</SUP>I-β-galactosidase, a low molecular weight degradation products were observed in plasma as revealed by gel filtration radio-chromatography.
- 日本薬物動態学会の論文
日本薬物動態学会 | 論文
- COMPETITIVE DISPLACEMENT OF SERUM PROTEIN BINDING TO REGULATE PHARMACOKINETICS OF A CEREBRAL BLOOD FLOW RADIOPHARMACEUTICAL
- Studies on the Metabolic Fate of Felodipine. (IV). Identification of New Metabolites of Felodipine in Rat.
- Metabolic Fate of Pergolide Mesylate. (2): Absorption, Distribution, Excretion of 14C-Pergolide Mesylate in Rats after Repeated Administration.
- Studies on the Metabolic Fate of 3H-TJN-101. (III): Absorption, Distribution and Excretion after Multiple Oral Administration to Rats.
- Studies on the Pharmacokinetics of Perindopril Erbumine in Rats. (1): Plasma Level Profile, Distribution, Metabolism and Excretion after Single Oral Administration.