Formation and Decomposition of Pyrophosphate Related to Bacterial Photophosphorylation
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1) When chromatophores from <I>Rhodospirillum rubrum</I> were illuminated with added [<SUP>32</SUP>P] P<SUB>i</SUB> and without added ADP, the radioactive P<SUB>i</SUB> was incorporated into some phosphate compounds. A small but definite amount of the compounds thus formed was adsorbed on charcoal, but not the remainder, most of which was purified and identified with pyrophosphate (PP<SUB>i</SUB>).<BR>2) The photosynthetic PP<SUB>i</SUB> formation required Me and was stimulated by oligomycin, whereas the photosynthetic ATP formation was stimulated by either Mg<SUP>2+</SUP> or Mn<SUP>2+</SUP> and inhibited by the antibiotic. The photosynthetic PP<SUB>i</SUB> formation in the presence of Mg<SUP>2+</SUP> was completely inhibited by Mn<SUP>2+</SUP>.<BR>3) Both the photosynthetic PP<SUB>i</SUB> and ATP formations were stimulated and depressed by 2, 6-dichlorophenol indophenol <I>plus</I> ascorbate or phenazine methosulfate, but the optimum concentrations of the redox-reagents were different for these two formations.<BR>4) The photosynthetic PPi formation was inhibited by ADP, antimycin A, <I>o</I>-phenanthroline, arsenate, and 2, 4-dinitrophenol but not by ATP. The maximum extent of the inhibition by ADP was approximately 40%.<BR>5) Well-washed chromatophores hydrolyzed PP<SUB>i</SUB> into P<SUB>i</SUB>. The pyrophosphatase [EC3. 6. 1. 1] activity was partially depressed in the light even under conditions where the photosynthetic PP<SUB>i</SUB> formation did not occur. The activity in darkness was inhibited by P<SUB>i</SUB> and arsenate, but not by ATP or ADP. It was stimulated by 2, 6-dichlorophenolindophenol, 2, 4-dinitrophenol, Li<SUP>+</SUP>, Na<SUP>+</SUP>, and K<SUP>+</SUP>.<BR>6) When chromatophores were depleted of the quinones, the pyrophosphatase activity in darkness decreased partially. When ubiquinone-10 was added, the activity was restored.<BR>7) By sonication of chromatophores, the pyrophosphatase activity in darkness increased to some extent.<BR>8) The mechanism of the photosynthetic PP<SUB>i</SUB> formation was discussed with relation to that of the photosynthetic ATP formation.
- 社団法人 日本生化学会の論文
社団法人 日本生化学会 | 論文
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