Redox Components Associated with Chromatophores from Rhodospirillum rubrum

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1. Light-grown cells of a blue-green mutant of Rhodospirillunt rubrrnn possess cytochrome c2, cvtochrome cc', cytochrome b-557.5 and cytochrome B. Approximately 73% to 9% of the total amounts of cytochrome c2 and cvtochrome cc' are present in the cvtoplasmic fluid, the remainder being associated with the chromatophore membrane, which contains cytochrome B also. It is not known whether cytochrome B is the cytochrome b-557.5 that has been purified. 2. With chromatophores, the cytochrome cc' is reduced by succinate, whereas the cytochrome B is reduced by NADH. The E_??_7 values of the cytochromes at the associated state are +0.311 V (n=1) for cvtochrome c2+0.017V (n=1) for cytochrome cc' and -0.16V (n=2) for cytochrome B. 3. The absorption spectrum of cytochrome cc' is significantly different between the free and the associated state. At the associated state, it is rather similar to that of the typical b-type cytochrome. 4. One chromatophorc of the average size possesses 790 molecules of bacterio-chlorophyll, 440 molecules of ubiquinone-10, 78 molecules of rhrdoquinone, 5 mole-cules of cytochrome c2, 5 molecules of cytochrome cc', 5 molecules of cytochrome B, 360 atoms of non-heme iron, 2 molecules of FAD, 1 molecule of FMMIN, and 3, 100 atoms of lipid phosphorus.
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