PREPARATION OF P32-LABELED ADENOSINE TRIPHOSPHATE BY HUMAN ERYTHROCYTES
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ATP32 labeled at β and γ position is easily prepared by incubating human erythrocytes with P32-orthophosphate. Optimal conditions for the method was studied. Need for neither buffer nor substrates and stability of the blood as a biological system are the main advantages of the present method. The recovery of P32 in ATP reached about 25 per cent and the ratio of the specific activity of α-phosphorus to that of β or γ-phosphorus was less than 1:20.
- 社団法人 日本生化学会の論文
社団法人 日本生化学会 | 論文
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