LIPIDE METABOLISM OF MYCOBACTERIUM TUBERCULOSIS AVIUM:I. ENZYMATIC SYNTHESIS OF HYDROXAMIC ACID FROM FATTY ACID AND ITS SPECIFICITY
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1. The conversion of fatty acids to the corresponding hydroxamic acid was demonstrated with crude extract from Mycobacterium tuberculosis avium (strain Takeo). 2. Of the acids serving as substrates for this enzyme, straight saturated butyric and valeric, dicarboxylic sebacic, and unsaturated oleic acids were the most effective. Marginal formation of hydroxamic acid was observed with formic, glycolic, glyoxylic, pelargonic, caprinic, capronic, glutaric, adipic, and pimelic acids, and with several amino acids. 3. The hydroxamic acid formation is strongly supported by the present data as the carboxyl activation. It is also evident that this formation is not esterase-, amidase-, or transpeptidase-catalyzed reaction. 4. All the results summarized in the present study can be explained by the following reactions: Fatty acid+labile phosphate (protein bound)→(acyl-phosphate intermediate)NH2OH→hydroxamic acid. The detailed function of protein bound labile phosphate remains to be clarified. The authors are indebted to Mr. H. Katayama and Mr. J. Tobari for technical assistance, and to Prof. Dr. S. Akabori for his valuable advice and encouragement during the cource of this study.
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社団法人 日本生化学会 | 論文
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