Characterization of phosphonoglycosphingolipids containing pyruvate: Localization in Aplysia nerve bundles.
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A phosphonoglycosphingolipid, designated as FGL-IIb, was first identified in nerve fibers of <I>Aplysia kurodai</I> by two-dimensional TLC (Abe, S. <I>et al. </I> (1986) <I>Biomed. Res.</I> 7, 47-51), and its chemical structure has been determined to be 3, 4-<I>O-</I>(1-carboxyethylidene) Galβ1→ 3GalNAcα1→3 (Fucα1→ 2)(2-aminoethylphosphonyl→6) Galβ1→4Glcβ1→1cemmide (Araki, S. et al., submitted). Cryostat and paraffin sections of the nervous tissue and skin of <I>Aplysia</I> were examined immunohistochemically with antiserum against FGL-IIb. With this antiserum, only nerve bundles were stained distinctly: nerve cells in ganglia and in subcutaneous and muscular tissues and other cell elements were not stained. From histochemical findings in cryostat sections pretreated with chloroform-methanol (2: 1, v/ v) and from the results of Western blot analysis of the nervous tissue, the staining was concluded to be due to glycolipid antigens. The antiserum reacted with FGL-IIb and other phosphonoglycosphingolipids named FGL-I, FGL-IIa, FGL-V, and F-9 on TLC plates. This reactivity of FGL-IIb was abolished by mild acid-methanol treatment, and the lost reactivity was recovered by alkaline hydrolysis. These findings suggest that the free carboxyl group of the pyruvic acid of FGL-IIb is essential for the immunological reaction and that all the glycolipids listed above have the same epitope as that of FGL-IIb. Immunohistochemical findings indicated that these glycolipids including FGL-IIb are localized specifically in nerve bundles of <I>Aplysia</I>.
- 社団法人 日本生化学会の論文
社団法人 日本生化学会 | 論文
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