Two assimilation pathways of C1-compound in Streptomyces sp. No. 239 during growth on methanol.

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Activities of hydroxypyruvate reductase, malate lyase, isocitrate lyase and hexose phosphate synthase were found in methanol-grown Streptomyces sp. No. 239. The presence of two C1-compound assimilation pathways, the serine and the pentose monophosphate pathways, should be possible in the organism. During the growth on methanol, the maximum activity of hydroxypyruvate reductase was observed at the early stage of growth phase, while a great activity of hexose phosphate synthase appeared at the middle of logarithmic phase. Sulfanilamide (1mM) elongated the lag period of the organism. It seems that the serine pathway actually operates, especially at the early stage of growth phase, and that at the active logarithmic phase, the carbon flow into cellular constituents from methanol mainly occurs via the pentose monophosphate pathway. The activity of hexose phosphate synthase which catalyzed condensation of formaldehyde with ribulose 5-phosphate was separated into 3-hexulose phosphate synthase and phospho-3-hexuloisomerase by an isoelectric focusing electrophoretic technique.
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