Isolation and Characterization fo Polysaccharide Produced by Rhodotorula glutinis K-24

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Rhodotorula glutinis K-24 produces a highly viscous polysaccharide in an acidic medium. The polysaccharide was separated from the supernatant of the culture broth by addition of acetone, and partially purified by ethanol precipitation. The partially purified polysaccharide, of which the hydrolysate gave three spots corresponding to fucose, galactose, and mannose on a thin-layer chromatoplate, seemed to be homogeneous in free boundary electrophoresis, but in ultracentrifugation, it gave two peaks, major and minor, on the sedimentation pattern. The minor component containing mannose was removed by the fractional precipitation with ethanol. The purified polysaccharide thus obtained was found to be a fucogalactan ([α]25D-38°) composed of L-fucose and D-galactose in a molar ratio of about 1:1. Partial hydrolysis revealed the presence of core galactan in it. The sedimentation velocity of the purified polysaccharide decreased rapidly with increasing concentration of polysaccharide above 0.5%. The sedimentation coefficient at zero concentration was 10.7×10-13 at 30°C in 1% NaCl aq.
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