Effect of Sclerin and 2, 4-Dinitrophenol on Formation of Aminoacyl-tRNA by Mouse Liver Enzyme
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Sclerin (SCL) enhanced the formation of aminoacyl-tRNA by mouse liver enzyme both in _??_ heterologous reaction with yeast tRNA and in a homologous reaction with mouse liver "pH 5 fraction" to which was added CTP. The effect of SCL has been found to involve the following two points of action; one is the stimulation of the formation of aminoacyl-AMP accompanied with the activation of inorganic pyrophosphatase activity in "pH 5 fraction, " and the other is an increase in the incorporation of AMP and CMP into the tRNA fraction. On the other hand, 2, 4-dinitrophenol (DNP) rather inhibited the pyrophosphatase activity, though its effect on aminoacyl-tRNA formation varied depending on the kind of amino acids and the reaction condition. It is assumed that SCL stimulates amino acid incorporation into the protein and cold TCA soluble fractions of liver tissue through the above-mentioned mechanism of action.
- 公益社団法人 日本農芸化学会の論文
公益社団法人 日本農芸化学会 | 論文
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