Evidence for a vertical displacement of intramembranous particles on the plasma membrane of Tetrahymena cells by a local anesthetic.

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We examined the effect of a local anesthetic, dibucaine, on the plasma membrane of <I>Tetrahymena pyriformis</I> strain NT-1 using freeze-fracture electron microscopy. Intramembranous particles (IMPs) were distributed homogeneously on the plasma membrane of untreated cells. But, when <I>Tetrahymena</I> cells had been treated with 1.3 mM dibucaine for 5 min at growth temperature, freeze-fracture micrographs of the plasma membrane showed marked alterations. Although IMPs showed an almost homogeneous distribution, their density was elevated markedly on the protoplasmic fracture (PF) face but greatly reduced on the exoplasmic fracture (EF) face. Areas around deciliated portions had a reverse IMP density distribution for the PF and EF faces. These results suggest that dibucaine induced vertical displace-ment of the IMPs in the plasma membrane.
日本細胞生物学会の論文