Morulate bodies in Actinophryid heliozoa: A fixation artefact derived from microtubules?

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The distribution and appearance of morulate bodies in the heliozoon <I>Actinophrys</I> sol is described using electron-microscopy. The morulate bodies occur around the sites typically occupied by microtubular axonemes in well-fixed cells. The incidence of morulate bodies increases when fixation pro-cedures which preserve axonemes poorly are adopted; and they are absent when microtubules are stabilized with Taxol. If axonemes are removed by pretreatment of the cell with cold and colchicine, the incidence of morulate bodies is greatly reduced.<BR>It is concluded that morulate bodies are associated with disassembly of axonemes and may be derived from the axonemes.
日本細胞生物学会の論文