Further Investigation of Some Inhibitors on Myogenic Differentiation:Mechanism of Inhibition with HMBA on Quail Myoblasts Transformed with Rous Sarcoma Virus.
スポンサーリンク
概要
- 論文の詳細を見る
To investigate the mechanism of myogenic differentiation, we are using quail myoblast cells (QM cells) transformed with a temperature-sensitive mutant of Rous sarcoma virus (ts-RSV), termed QM-RSV cells. At 35.5°C, a permissive temperature for RSV, QM-RSV cells repeatedly proliferate without differentiation, but, at 41°C, a nonpermissive temperature, myogenic differentiation proceeds. This temperature dependency of the differentiation is derived from protein kinase activity of pp60v-src, as tyrosine dephosphorylation is necessary for myogenic differentiation of QM-RSV cells. In this study, it was demonstrated that among four fusion inhibitors, aspirin, doxorubicin, HMBA and TPA, three of the inhibitors, except for TPA, inhibited inyogenin gene expression. Moreover, HMBA inhibited myoblast fusion accompanying inhibition of tyrosine dephosphorylation of certain proteins, and recovered the tyrosine kinase activity of pp60v-src to a certain extent. To study the effect of HMBA on the intracellular localization of pp60v-src, detergent-soluble and detergent-resistant fractions were prepared with Triton X-100. As a result, it was shown that pp60v-src mainly exists in detergent-resistant fraction at 35.5°C. While almost all of the pp60v-src at 41°C exists in detergent-soluble fraction. HMBA treatment retained pp60v-src in detergent-resistant fraction even at 41°C. These results suggest that HMBA inhibits myogenic differentiation of QM-RSV cells by affecting the regulation of pp60v-src.
- 日本細胞生物学会の論文
日本細胞生物学会 | 論文
- テトラヒメナにおけるDNA-核膜複合体の研究 (細胞核内小器官の生物学)
- 核小体におけるリボゾ-ムRNA合成の制御 (細胞核内小器官の生物学)
- 細胞分裂とその調節-分裂装置をめぐって (細胞増殖と分化)
- 細胞雑種研究の現状 (細胞融合)
- 浮遊増殖性癌細胞の無血清培養と培地添加アルブミンの役割 (細胞融合)