Golgi Membrane Vesicles in HeLa Mitotic Cells are Identified with Monoclonal Antibody Made against Golgi Cisternal Membrane Protein p138.
スポンサーリンク
概要
- 論文の詳細を見る
A monoclonal antibody (mAbG3A5) recognizing p138 antigen was used to identify the Golgi cisternal membrane and determine behavior of Golgi fragments during mitosis in HeLa cells. At the start of mitosis, Golgi stacks identified with the mAbG3A5 antibody were fragmented into fine membrane vesicles which were distributed throughout the cytoplasm leaving only the region of the chromosome cluster unoccupied. On Western immunoblotting analysis, p138 was found associated with the membrane fraction prepared from mitotic HeLa cells having a buoyant density the same as that of interphase Golgi membranes. In addition to the fine Membrane vesicles, clusters labeled with mAbG3A5 antibody were frequently observed in mitotic cells. They Numbered 11 on average per mitotic cell and consisted of fine membrane vesicles of which membrane region was labeled with the mAbG3A5 antibody. This fact indicates that the membrane vesicles in mitotic Golgi clusters were also part of the fragments of Golgi cisternae. The number of mitotic Golgi clusters per mitotic cell was constant from prophase to anaphase, increasing twofold at telophase, although the average size of mitotic Golgi cluster remained unchanged throughout mitosis. The increase in number of mitotic Golgi clusters at telophase was accompanied by decrease in immunofluorescence of fine membrane vesicles. Treatment with nocodazole caused the disappearance of the mitotic Golgi clusters from prophase cells; however upon removal of it, they were reformed. These results suggest that during mitosis the Golgi apparatus were fragmented to fine membrane vesicles leaving only a part as mitotic Golgi clusters and were reassembled through tentative clustering of the fine membrane vesicles at the end of mitosis.The Golgi apparatus of mammalian cells is comprised of several stacks arranged in a circumscribed juxtanuclear region to which the microtubule organizing center localizes (Thyberg and Moskalewski, 1985). Studies using electron microscopy and immunocytochemical procedures have shown that Golgi apparatus are fragmented and dispersed throughout the cytoplasm in mitotic cells (Zeligs and Wollman, 1979; Lucocq et al, 1987). The fragmentation of Golgi apparatus during mitosis.
- 日本細胞生物学会の論文
日本細胞生物学会 | 論文
- テトラヒメナにおけるDNA-核膜複合体の研究 (細胞核内小器官の生物学)
- 核小体におけるリボゾ-ムRNA合成の制御 (細胞核内小器官の生物学)
- 細胞分裂とその調節-分裂装置をめぐって (細胞増殖と分化)
- 細胞雑種研究の現状 (細胞融合)
- 浮遊増殖性癌細胞の無血清培養と培地添加アルブミンの役割 (細胞融合)