Successful Development of Air-Dried Microplates (HP-Plates) for Susceptibility Testing against Helicobacter pylori Isolates
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We have successfully developed and evaluated a new susceptibility testing procedure against Helicobacter pylori strains using air-dried microplates "HP-Plates" containing eight serially-diluted anti-H. pylori agents. HP-Plate wells were reconstituted by the inoculation of 100μl of H. pylori cell suspensions. After incubation at 37C for 48hr under humidified microaerophilic conditions, HP-Plates were read visually with a circular mirror. We investigated the within-day reproducibility tests of HP-Plates using the six quality control (QC) strains we proposed. Of the 20 testings, determining the minimum inhibitory concentrations (MICs) of all the QC strains fell within ±1 log2 dilution ranges. When 200 clinical isolates were tested with HP-Plates and compared with the results obtained with the modified broth macrodilution method of NCCLS, more than 90% of the MICs also fell within ±1 log2 dilution ranges. We concluded that the HP-Plate susceptibility test method is a practical and easily applicable alternative of susceptibility testing for clinical microbiology laboratories in determining the MICs of H. pylori isolates.
- 微生物学・免疫学学会連合の論文
微生物学・免疫学学会連合 | 論文
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