In Vitro Amphotericin B Effects on Growth, Viability and Dimorphism of Paracoccidioides brasiliensis
スポンサーリンク
概要
- 論文の詳細を見る
The in vitro effects of amphotericin B deoxycholate suspension (fungizone) on Paracoccidioides brasiliensis growth, cell viability and transformation were investigated. We also analyzed the protein synthesis patterns of both cellular forms, yeast and mycelium in the presence of AmB. This drug, at 30μg/ml, highly inhibited yeast growth, which could be recovered depending on treatment time, where the most effective reversion was observed after 6hr of incubation. The yeast cell viability, that had been partially affected by the drug, could also be efficiently recovered after AmB was removed. The effect of AmB on the cellular dimorphism process showed a strong reduction in the mycelium to yeast transformation (80% inhibition compared to the control without the drug). On the other hand, the transformation from yeast to mycelium in the presence of AmB was 50% affected, relative to the control. In contrast to the growth and cell viability experiments, the reversion effects on dimorphism were partial when the drug was removed, even with only 6hr treatment. The two-dimensional gels of 35S-labeled proteins revealed a strong reduction in the three species of 80, 71 and 56kDa in yeast and mycelium when treated with AmB.
- 微生物学・免疫学学会連合の論文
微生物学・免疫学学会連合 | 論文
- The Structural Proteins of Newcastle Disease Virus
- The Pathogenicity of Newcastle Disease Virus Isolated from Migrating and Domestic Ducks and the Susceptibility of the Viral Glycoproteins to Proteolytic Cleavage
- Analysis of Nuclear Accumulation of Influenza Nucleoprotein Antigen Using a Temperature-Sensitive Mutant
- Enhancement of fusion from within by Antiviral Antibody in Cells Infected with Newcastle Disease Virus
- Analysis of Nuclear Accumulation of Influenza Nucleoprotein Antigen in the Presence of p-Fluorophenylalanine