The Non-Random Pattern of Insertion of IS2 into the hemB Gene of Escherichia coli
スポンサーリンク
概要
- 論文の詳細を見る
The hemB gene of Escherichia coli has been identified as a hot spot for the insertion of the transposable element IS2. The insertional specificity of IS2 is still unclear. This study reports on the attempt to sequence a statistically significant number of insertions in hemB, in order to determine whether there might be a basis for future studies to determine a molecular basis of IS2 insertional specificity. The results indicate that IS2 inserts in a non-random manner into a 240bp segment at the 5' end of the gene (region I). Twenty-one of 24 insertions occurred in region I. Three insertions have been identified in the two middle 250bp segments of the 975bp gene, and none in the 3' terminal segment. A seventeen bp sequence showing 88.2% identity with a segment of IS2, 221bp from the 3' terminus has been identified in region I. Four instances of repeated insertion between the same pair of nucleotides have been observed at four different sites.
- 微生物学・免疫学学会連合の論文
微生物学・免疫学学会連合 | 論文
- The Structural Proteins of Newcastle Disease Virus
- The Pathogenicity of Newcastle Disease Virus Isolated from Migrating and Domestic Ducks and the Susceptibility of the Viral Glycoproteins to Proteolytic Cleavage
- Analysis of Nuclear Accumulation of Influenza Nucleoprotein Antigen Using a Temperature-Sensitive Mutant
- Enhancement of fusion from within by Antiviral Antibody in Cells Infected with Newcastle Disease Virus
- Analysis of Nuclear Accumulation of Influenza Nucleoprotein Antigen in the Presence of p-Fluorophenylalanine