Cloning and Expression of cDNAs from Enterically-Transmitted Non-A, Non-B Hepatitis Virus

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• The fragment gene of enterically-transmitted non-A, non-B hepatitis virus (ET-NANBHV) was cloned as a cDNA and inserted into an expression vector pUEX2. The recombinant protein was expressed in Escherichia coli HB101 as a fusion protein with β-galactosidase (β-Gal). The fusion protein reacted with the sera of infected cynomolgus monkeys and of patients from Myanmar. This reaction was highly related with ET-NANBHV infection, and obviously demonstrates in that the recombinant protein can be used for the detection of ET-NANBHV infection.

• 微生物学・免疫学学会連合の論文

微生物学・免疫学学会連合 | 論文

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